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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209722

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_C4341S

Plasmid

#187156

Purpose

mammalian cell expression FLAG-HUWE C4341S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

C4341S

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pACRISPR-KM

Plasmid

#203587

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Article

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PKC alpha DN

Plasmid

#21235

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K368R

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

Plasmid

#214885

Purpose

Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra2

Plasmid

#179479

Purpose

Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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JUP_pLX307

Plasmid

#98347

Purpose

Lentiviral expression of JUP

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

JUP (JUP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 WT-T2A-GFP

Plasmid

#163787

Purpose

Expression of Wild-type CARD11

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MND

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Article

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FOSL2-P2A-mCherry

Plasmid

#203860

Purpose

Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

FOSL2 (FOSL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-PC

Plasmid

#92125

Purpose

Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Tags

Expression

Mutation

Promoter

Ppdc

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLVE-hTGFB3-IRES-Red

Plasmid

#52580

Purpose

2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

Depositor

Charles Gersbach

Article

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

human transforming growth factor-beta3 (TGFB3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-pelB

Plasmid

#157740

Purpose

empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Use

Tags

PelB signal sequence and possible His6-tag (adds …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

Plasmid

#210523

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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