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Plasmid#141247PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTL-Bag2T-gRNA2
Plasmid#196253PurposePlasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TEV-12His
Plasmid#137032Purposeempty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)DepositorTypeEmpty backboneUseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008
Plasmid#187918PurposeExpresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 1
UseTags3xFLAGExpressionMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-R9-GFP-HiBit
Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-eCFP
Plasmid#183155PurposePlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFPDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA1
Plasmid#158479PurposeEntry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationSTOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCPP5108
Plasmid#128726PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5107
Plasmid#128725PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-2.01 beta
Plasmid#69591PurposeOT-II TCR beta chain cDNA in pCRIIDepositorInsertTCR beta clone 1.1 (Tcrb Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_Zeo_3xflag_Luciferase
Plasmid#136533PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
UseTagsExpressionBacterialMutationSynonymous point mutation in the wobble position…PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA0.002
Plasmid#119560PurposeLevel 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJA3
Plasmid#19547DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
BB3aN_14*
Plasmid#98548Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-2x
Plasmid#164414PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
E2_pENTR_R4R3_T CTP
Plasmid#171736PurposeptpTALECD vector assembly, Entry clone 2 with attR4 and R3DepositorInsertA terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only