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ATP9A-luciferase

Plasmid

#113354

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ATP9A-enhancer (ATP9A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAG415-ADH1p-HA-rap1-K9R

Plasmid

#84531

Purpose

This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

ADH1

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab11 CA

Plasmid

#53472

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

Q70L

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pMT3-RhoGC E254D

Plasmid

#100033

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC E254D

Use

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E254D

Promoter

Adenovirus major late promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab32 DN

Plasmid

#37739

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T33N (dominant negative)

Promoter

GAL4

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pIS1-mutant RhoA 3'UTR

Plasmid

#26090

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant RhoA 3'UTR (RHOA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Both miR-31 binding sites in 3’ UTR mutagenized. …

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab32 CA

Plasmid

#37740

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

GAL4

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_BFP

Plasmid

#45302

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pBa.FRB-3myc-KIF13AS1371A tail 361-1749

Plasmid

#224411

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Use

Tags

FRB-3myc

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His6, LgBiT

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

AVI tag and His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IZU-LAMP1-mRuby3

Plasmid

#222586

Purpose

Stable expression of LAMP1-mRuby3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LAMP1 (LAMP1 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-LC3

Plasmid

#221738

Purpose

Stable expression of mRuby3-LC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LC3B (Map1lc3b Rat)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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