Addgene

We narrowed to 13,597 results for: pET

Showing: 6861 - 6880 of 13597 results

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pDEST-FRT/T0-GFP-PALB2

Plasmid

#71113

Purpose

mammalian expession of GFP-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-Cas9-162A

Plasmid

#84918

Purpose

for in vitro transcription of Cas9 with polyA tail encoded

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Cas9

Use

Tags

Flag, NLS, and polyA tail

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-LacR-PALB2

Plasmid

#71112

Purpose

mammalian expession of mCherry-LacR-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124603

Purpose

Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Has Service

AAV9

Insert

Soma-localized ChrimsonR

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee

Plasmid

#134775

Purpose

mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee

Use

Tags

FLAG and mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCLX-CHOP-dGFP

Plasmid

#71299

Purpose

Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CHOP (ATF-responsive)

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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His-DERA

Plasmid

#203225

Purpose

For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

Depositor

Vern Schramm

Article

Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

Insert

deoxyribose aldolase

Use

Tags

N-terminal TEV cleavable 6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Marathon-RT tetramutant for Split-PE (pEH101)

Plasmid

#190109

Purpose

Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Use

Tags

bpNLS

Expression

Mammalian

Mutation

D14R, D74R, N116K, N197R

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zeb2-3'UTR

Plasmid

#61771

Purpose

Luciferase reporter containing the 3'UTR of mouse Zeb2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zeb2 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
plc242-GFP-WDR24

Plasmid

#87050

Purpose

mammalian expression of WDR24

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

WDR24 (WDR24 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTrc-trGPPS(CO)-LS

Plasmid

#50603

Purpose

pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

Depositor

Jay Keasling

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Inserts

Limonene Synthase
ApR
LacIq

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

truncated codon optimized geranylpyrophosphate sy…

Promoter

Trc

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-3xFlag-ETO

Plasmid

#12507

Depositor

Dong-Er Zhang

Article

Biggs et al Mol Cell Biol. 2006 Oct . 26(20):7420-9.

Insert

ETO (RUNX1T1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pMDH

Plasmid

#133129

Purpose

A recombinant plasmid expressing mdh (malate dehydrogenase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

malate dehydrogenase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

malate dehydrogenase promoter

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP

Plasmid

#71108

Purpose

mammalian expession of Flag-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

TRE promoter, Tet ON

Available since

Nov. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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Rogi2-CMV-LAMB3-T2A-GFP-bGH

Plasmid

#179865

Purpose

Targets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFP

Depositor

George Church

Article

Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

Insert

LAMB3-T2A-GFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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dABE

Plasmid

#124447

Purpose

Expresses dead Cas9 (dCas9)-ABE7.10

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dABE7.10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pconst #5-RFP

Plasmid

#81079

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23110

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Use

Tags

Expression

Mammalian

Mutation

R1608Q

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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