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Plasmid#202527PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CALID
Plasmid#169708PurposeCre-activated light-inducible Dre recombinaseDepositorInserta double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB431
Plasmid#68696PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (S25N)
Plasmid#184036PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationSerine 25 changed to AsparaginePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB225
Plasmid#68616PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b_Q69L
Plasmid#186272PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis-tagged at N terminalExpressionBacterialMutationChanged Q69 into LPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC267
Plasmid#118887Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tagDepositorInsertNcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; HALO tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB406
Plasmid#68671PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
FusionRed-VASP-5
Plasmid#56136PurposeLocalization: Focal Adhesions, Excitation: 580, Emission: 608DepositorInsertVASP
UseTagsFusionRedExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-CAG-NCreIntN-EF1a-mTagBFP2
Plasmid#160507PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RapR-FAK-YM-KD
Plasmid#25929DepositorInsertRapR-FAK (Ptk2 Human, Mouse)
UseTagsEGFPExpressionMammalianMutationThis pEGFP-RapR-FAK-YM-KD plasmid contains the FA…PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB215
Plasmid#68606PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1
Plasmid#86816Purposemammalian expression of lncRNA LINC00673/SLNCR1DepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + codon optimized Brn3a
Plasmid#86829Purposemammalian expression of the transcription factor Brn3aDepositorInsertBrn3a (POU4F1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-Smad2 EPSM
Plasmid#14933DepositorInsertSmad2 EPSM (SMAD2 Human)
UseTagsFlagExpressionMammalianMutationErk/Pro-directed kinase site mutant (EPSM). T220V…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(K433E)
Plasmid#42514DepositorInsertPyruvate Kinase M2 Lys433->Glu
UseRetroviralTagsFlagExpressionMutationChanged lysine 433 to glutamatePromoterAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
SI16-Kit a
Plasmid#58948Purposeexpresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cellsDepositorInsertKit receptor a mouse monoclonal antibody (kita Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only