-
Plasmid#183151PurposePlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only
-
-
pC0.053
Plasmid#119571PurposeLevel 0 part. PromoterDepositorInsertPnirA
UseSynthetic BiologyTagsExpressionMutationRBS* added (22 bp) upstream of ATG.PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8
Plasmid#169962PurposepDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-nCas9n-P2A-TagBFP2-pA
Plasmid#176026PurposeMultisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENTDepositorInsertnCas9n-P2A-TagBFP2
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJA2
Plasmid#19546DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRai
Plasmid#189938PurposeExpresses CRISPRai cassette under EF1a promoterDepositorInsertCRISPRai cassette
UseTagsExpressionMammalianMutationPromoterCMV enhancer-rat EF1 alphaAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJA3
Plasmid#19520DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
UseTagsV5ExpressionMammalianMutationF193L based on NP_005138.3PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAT.015
Plasmid#119558PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PresentER-MSIIFFLPL (GFP)
Plasmid#102946PurposePresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)DepositorInsertMSIIFFLPL (Serpinf1 Synthetic, Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJB9
Plasmid#19532DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_Luciferase
Plasmid#136528PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only