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Plasmid#103218PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130b-5p target (MIR130B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-130b-3p
Plasmid#103217PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130b-3p target (MIR130B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(N)-SH2
Plasmid#46470DepositorInsertPLCg1(N)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 539-664PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 DN
Plasmid#44831DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS25N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX BRDG1-SH2
Plasmid#46410DepositorInsertBRDG1 (STAP1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 167-283PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Vav3-SH2
Plasmid#46530DepositorInsertVav3 (Vav3 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 660-774PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JARID2-KO-gRNA-1
Plasmid#131328PurposegRNA to knockout JARID2. Use with JARID2-KO-gRNA-2DepositorInsertJARID2-KO-gRNA-1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JARID2-KO-gRNA-2
Plasmid#131327PurposegRNA to knockout JARID2. Use with JARID2-KO-gRNA-1DepositorInsertJARID2-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-5p
Plasmid#103166PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP251 pEF1a-UNC5C-4-SunTag
Plasmid#100940PurposeTALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO geneDepositorInsertTALE target sequence for UNC5C, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab4aM-7
Plasmid#56441PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab4a (RAB4A Human)
UseTagsEGFPExpressionMammalianMutationMutation removes NheI sitePromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LSB-hsa-miR-551b-3p
Plasmid#103642PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-551b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-551b-3p target (MIR551B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-200c-3p
Plasmid#103332PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-200c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-200c-3p target (MIR200C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-1-3p
Plasmid#103722PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-1-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-16-5p
Plasmid#103272PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-16-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-16-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-103a-3p
Plasmid#103168PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-103a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-103a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29b-3p
Plasmid#103394PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-204PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-152-3p
Plasmid#103260PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-152-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-152-3p target (MIR152 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only