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Plasmid#193937PurposeMammalian expression of GFP-tagged Kif 3C DD 616-796DepositorInsertKif 3C (Kif3c Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KAP3B
Plasmid#193940PurposeMammalian expression of Halo-tagged KAP 3BDepositorInsertKAP 3B (KIFAP3 Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B CC 391-592
Plasmid#193930PurposeMammalian expression of GFP-tagged Kif 3B CC 391-592DepositorInsertKif 3B (Kif3b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C CC 411-615
Plasmid#193935PurposeMammalian expression of GFP-tagged Kif 3C CC 411-615DepositorInsertKif 3C (Kif3c Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B CC 391-592
Plasmid#193931PurposeMammalian expression of Halo-tagged Kif 3B CC 391-592DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B DD 593-747
Plasmid#193933PurposeMammalian expression of Halo-tagged Kif 3B DD 593-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3A DD 590-702
Plasmid#193927PurposeMammalian expression of GFP-tagged Kif 3A DD 590-702DepositorInsertKif 3A (KIF3A Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3A DD 590-702
Plasmid#193928PurposeMammalian expression of Halo-tagged Kif 3A DD 590-702DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3A CC 419-589
Plasmid#193925PurposeMammalian expression of GFP-tagged Kif 3A CC 419-589DepositorInsertKif 3A (KIF3A Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep:Luc
Plasmid#192987PurposePlasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationRepressor element deletedPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
UseTagsmScarlet fused to FKBPExpressionYeastMutationPromoterSP6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_-6D
Plasmid#149387PurposeP. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation6bp deleted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_-5D
Plasmid#149388PurposeP. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation5bp deleted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+5D
Plasmid#149390PurposeP. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation5bp inserted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D
Plasmid#149391PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Opt
Plasmid#149395PurposeP. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is optimized at key positions needed…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine
Plasmid#186418PurposeExpression of scrambled shRNA used as a control for knock down experimentsDepositorInsertScramblemiR
UseAAVTagsmCitrineExpressionMutationPromoterhuman Synapsin1Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine
Plasmid#186419PurposeExpression of shRNA for the knock down of mouse Egr3DepositorInsertmiREgr3-5D
UseAAVTagsmCitrineExpressionMutationPromoterhuman Synapsin1Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-TsCC(B)::KANMX
Plasmid#176397PurposePlasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.DepositorInsertmScarlet-TsCC(B)
UseTagsmScarlet fused to TsCC(B)ExpressionBacterial and YeastMutationPromoternoneAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only