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Showing: 6841 - 6860 of 300918 results

  2. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-434022

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  3. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-434022

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  10. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224898-plasmid-map-sequence-id-443622

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  17. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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  18. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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  19. TFORF1974

    Plasmid
    #143988
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX3 (TBX3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143988-plasmid-map-sequence-id-385902

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  20. TFORF1974

    Plasmid
    #143988
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX3 (TBX3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143988-plasmid-map-sequence-id-385902

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Showing: 6841 - 6860 of 300918 results