Addgene

We narrowed to 38,744 results for: CMV

Showing: 6821 - 6840 of 38744 results

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pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Expression

Mutation

Promoter

CMV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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YE2-BE4max

Plasmid

#138156

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE2)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CRBN-P2A-Hygro

Plasmid

#124303

Purpose

Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CRBN (CRBN Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Nuc-meCpG

Plasmid

#186464

Purpose

Expresses mCherry tagged methyl-CpG recognition motif targeted to nucleus in mammalian cells.

Depositor

Chongli Yuan

Article

Zhao et al ACS Sens. 2021 Oct 22;6(10):3575-3586. d

Insert

dimeric MBD1 (MBD1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2A_tdTomato

Plasmid

#184045

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Y701F-STAT1

Plasmid

#71453

Purpose

Lentiviral expression of Y701F-STAT1

Depositor

George Stark

Article

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Y701F

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
pCDH_SBP-EGFP-Ecadherin

Plasmid

#65290

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_pCDest

Plasmid

#119923

Purpose

Nme2Cas9 CMV-driven mammalian expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

Tags

NLS-3xHA-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE1-2

Plasmid

#219945

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end

Use

CRISPR

Tags

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)

Plasmid

#132546

Purpose

Expresses CIRTS-10 (bdefensin 3-SLBP-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE3-4

Plasmid

#219946

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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EE-BE4max

Plasmid

#138158

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(EE)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 R126E + R132E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Plasmid

#132544

Purpose

Expresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-EGFP

Plasmid

#65282

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-CP1028

Plasmid

#138160

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-CUL4A WT-P2A-Puro

Plasmid

#124304

Purpose

Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-Ecadherin

Plasmid

#65291

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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