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Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of nuclear lacz reporterTagsNuclear localization signalExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-pET151
Plasmid#177870PurposeExpresses mouse Talin 1 F2 domain in bacterial cellsDepositorInsertmouse Talin 1 F2 domain (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-pET151
Plasmid#177877PurposeExpresses mouse Talin 1 F2F3 domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 domain (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_316
Plasmid#180547PurposeEntry vector containing sfGFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_344
Plasmid#180548PurposeEntry vector containing cjBlueDepositorInsertcjBlue
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_345
Plasmid#180550PurposeEntry vector containing eforRedDepositorInserteforRed
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_009
Plasmid#180518PurposeEmpty backbone for D1.1 cloning, contains LacZ dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_137
Plasmid#180521PurposeEmpty backbone for D1.2 cloning, contains sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_010
Plasmid#180519PurposeEmpty backbone for D1.2 cloning, contains LacZ dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_048
Plasmid#180564PurposeEntry vector containing L3S1P00DepositorInsertL3S1P00_E1.4b
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_033
Plasmid#180545PurposeEntry vector containing LuxRDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_016
Plasmid#180523PurposeEmpty backbone for D2.2b cloning, contains LacZ dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_015
Plasmid#180522PurposeEmpty backbone for D2.2a cloning, contains LacZ dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)
Plasmid#172064PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intronDepositorInsertmTagBFP2(11x7)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)
Plasmid#172065PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intronDepositorInsertmTagBFP2(11x7)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11
Plasmid#172068PurposeProtein trap plasmid for inserting GFP11 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pColE1-AlphaOmega
Plasmid#165867PurposeTerminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only