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Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2A-SH2
Plasmid#46481DepositorInsertSH2A (SH2D4A Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 338-445PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-EB3-7
Plasmid#57953PurposeLocalization: MT End Binding Protein, Excitation: 549, Emission: 565DepositorInsertEB3 (MAPRE3 Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
TOP2A sgRNA1
Plasmid#138190Purpose3rd generation lentiviral gRNA plasmid targeting human TOP2ADepositorInsertTOP2A (TOP2A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOP2A sgRNA2
Plasmid#138191Purpose3rd generation lentiviral gRNA plasmid targeting human TOP2ADepositorInsertTOP2A (TOP2A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172669PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertRNF2 pegRNA and RNF2_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP_3xFlag-Myc-CMV26
Plasmid#73577PurposeExpress mouse OC-STAMP in mammalian cellsDepositorInsertOC-STAMP (Ocstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag-RARalpha (T181A,S445A,S461A)
Plasmid#35556DepositorInsertRARalpha (RARA Human)
UseTagsFlag and HisExpressionMammalianMutationT181A, S445A, S461APromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCMV5 Flag Jnk1a1(APY)
Plasmid#15742DepositorInsertJnk1a1(TPF) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationThr-190AlaPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap-SH2
Plasmid#46500DepositorInsertSlap (SLA Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 61-221PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-100-5p
Plasmid#103164PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-100-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-100-5p target (MIR100 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP250 pEF1a-TALE-SunTag
Plasmid#100939PurposeEmpty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO geneDepositorInsertTALE insertion site, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SLC7A11 sh926R
Plasmid#118701Purposeentry clone for SLC7A11 insensitive to hairpin TRCN0000288926DepositorInsertSLC7A11 (SLC7A11 Human)
UseTagsExpressionMammalianMutationwt , SLC7A11 sh926RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat4-SH2
Plasmid#46514DepositorInsertStat4 (STAT4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 133-678PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-210-3p
Plasmid#103346PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-210-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-210-3p target (MIR210 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-210-5p
Plasmid#103347PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-210-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-210-5p target (MIR210 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133a-3p
Plasmid#103221PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only