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pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138318

Purpose

Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SauCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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SFG.CNa22_opt.IRES.eGFP

Plasmid

#22491

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised Calcineurin A mutant 22 (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

V314R; Y341F

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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mOrange2-Paxillin-22

Plasmid

#57970

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
RRAS2

Plasmid

#55660

Purpose

expression of mouse RRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS2 (Rras2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Article

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pMPRMA38

Plasmid

#172937

Purpose

pQE30 encoding Wzz mutant i92

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i92 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA45

Plasmid

#172934

Purpose

pQE30 encoding Wzz mutant i32

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i32 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA48

Plasmid

#172941

Purpose

pQE30 encoding Wzz mutant i131

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i131 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA50

Plasmid

#172936

Purpose

pQE30 encoding Wzz mutant i219

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i219 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-mKate2-myc no-pA

Plasmid

#80812

Purpose

myc epitope fused to C-terminus of mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mKateHA p3E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB6577

Plasmid

#106129

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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miR-206-1/miR-133b promoter GFP

Plasmid

#20793

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-206-1/mir-133b (mir206-1 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pBAD-CAR-GECO1

Plasmid

#113939

Purpose

Bacterial expression of red fluorescent Ca2+ sensor CAR-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

CAR-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-206 site

Plasmid

#25027

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-206 site

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pRK5 Flag-RagC(L91P)

Plasmid

#99726

Purpose

Mammalian expression of Flag-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

L91P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(K84T)

Plasmid

#99727

Purpose

Mammalian expression of Flag-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

K84T

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagA(R37P)

Plasmid

#99717

Purpose

Mammalian expression of Flag-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

Flag

Expression

Mutation

R37P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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POT5-TEF2p-ycrtYB-ADH1t

Plasmid

#65359

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtYB-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TEF2p-ycrtE-ADH1t

Plasmid

#65353

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtE-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET-32M human DKK1c (183–266)

Plasmid

#132626

Purpose

expresses human DKK1c (183–266) in E. coli

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

DKK1 (183–266)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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