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Plasmid#111862PurposeC-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDN(1-400)-GFP (Bicd2 Mouse)
UseTags8xHis tag, GFP, and ZZ tagExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-VMA Model vector
Plasmid#86590PurposeThis can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapIDepositorInsertVMA intein
UseTagsHis6-MBPExpressionBacterialMutationOriginal SapI site from pMal was mutated outPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-mcherry-WIPI2d-cs-TEV-STREP
Plasmid#178912PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d
UseTagsC term strep and N term mcherryExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-NSP10
Plasmid#154409PurposeGateway-compatible Entry vectorDepositorInsertNSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP7
Plasmid#154406PurposeGateway-compatible Entry vectorDepositorInsertNSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)
Plasmid#101105PurposeEncodes dCas9(C) (without VP64) fused to VEGFR1DepositorInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-NSP9
Plasmid#154408PurposeGateway-compatible Entry vectorDepositorInsertNSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-RNAi-nucCHERRYmiR-TEV-FLAG
Plasmid#105813PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-ZhcD-Cit-tevs-DHFR
Plasmid#116051PurposeReporter plasmid for measuring protease activityDepositorInsertZhcD-Cit-tevs-DHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-ORF7B
Plasmid#154422PurposeGateway-compatible Entry vectorDepositorInsertORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-ORF6
Plasmid#154420PurposeGateway-compatible Entry vectorDepositorInsertORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 7, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-ORF7A
Plasmid#154421PurposeGateway-compatible Entry vectorDepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-ORF8
Plasmid#154423PurposeGateway-compatible Entry vectorDepositorInsertORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP15
Plasmid#154413PurposeGateway-compatible Entry vectorDepositorInsertNSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
Plasmid#74053Purposeretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-tvD-Cit-tevs-DHFR
Plasmid#116053PurposeReporter plasmid for measuring protease activityDepositorInserttvD-Cit-tevs-DHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LIC4B-His-TEV-TAK1-TAB1
Plasmid#154874PurposeInsect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91DepositorInsertTAK1-TAB1 (TAB1 Human)
UseTagsLIC TEV HisExpressionInsectMutationCodon optimizedPromoterpolyhedrinAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_cx
Plasmid#167273PurposeOverexpression of crystallizing SNAP-tag variant (P179R) in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationP179RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-204PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-NSP8
Plasmid#154407PurposeGateway-compatible Entry vectorDepositorInsertNSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits