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pGBKT7-PRP5
Plasmid#87592PurposeEncodes Y2H Gal4 DNA binding domain for Prp5DepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TYK2
Plasmid#67133PurposeStandard for cell-free expression benchmarking.DepositorInsertTYK2 (TYK2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationA435SPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0021
Plasmid#117532PurposeLevel0 Golden Gate part: CDS, SaCas9 (no stop codon)DepositorInsertSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K740R
Plasmid#111953PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutationDepositorInsertHsh155 K740R
UseTagsExpressionBacterial and YeastMutationK740RPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
2xDHFR-myc
Plasmid#20215DepositorInsert2xDHFR (folA E. coli)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2A13
Plasmid#67103PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2A13 (CYP2A13 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT6
Plasmid#67123PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT6 (STAT6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP3
Plasmid#67083PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP3 (TFDP3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP1
Plasmid#67084PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP1 (TFDP1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP2
Plasmid#67088PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP2 (TFDP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFF1
Plasmid#67047PurposeStandard for cell-free expression benchmarking.DepositorInsertTFF1 (TFF1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-3xFRB
Plasmid#20223DepositorInsert3xFRB (MTOR Human)
UseTagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-LOVPLD-mCherry-OSBP(PH)
Plasmid#219561PurposeGolgi-targeted LOVPLDDepositorInsertLOVPLD-mCherry-OSBP(PH)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-lyn10-mCherry-dLOVPLD
Plasmid#219556PurposePM-targeted catalytically dead LOVPLDDepositorInsertlyn10-mCherry-dLOVPLD
UseTagsmCherryExpressionMammalianMutationH170A mutation on PLDPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-p18-mCherry-LOVPLD
Plasmid#219560Purposelysosome-targeted LOVPLDDepositorInsertp18-mCherry-LOVPLD
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-24XGP41-J9
Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-FRB
Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FRB-dCer3-CAAX
Plasmid#191326PurposeExpresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-dCer-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only
