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Plasmid#160656PurposeExpresses codon optimized SARS-CoV-2 NSP8 in E. coliDepositorInsertSARS-CoV-2 NSP8 (ORF1ab Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGC4593
Plasmid#110206PurposeAccessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)DepositorInsertsTEV protease
aaRS
UseTagsExpressionBacterialMutationPromoterPbad and PtetAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB207
Plasmid#164122PurposeJ23101 driving PP7 repeatsDepositorInsertYFP-PP7
UseTagsExpressionBacterialMutationPromoterJ23101Available sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cb5-IRES-puromycin-pLVx-EF1a
Plasmid#177434PurposeTo express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.DepositorInsertCb5 tail anchor (Cyb5r4 Rat)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4-Y801H Ankyrin Repeats pET151
Plasmid#164058PurposeExpresses mouse KANK4 Ank domain -Y801H mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
Pooled Library#138015PurposeThis library can be used to investigate cardiac gene regulation. It targets transcriptional regulators that are expressed in mouse cardiomyocytes. A subset of GATA4 regulated genes are also included.DepositorSpeciesMus musculusUseAAV, CRISPR, and Cre/LoxAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Esyn-FlpX-TVA950-EYFP-WPRE
Plasmid#234710PurposepAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFPDepositorInsertTVA950-eYFP
UseAAV; Has fdio cassette for flp-dependent expressi…TagsExpressionMammalianMutationPromoterenhanced SynapsinAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3396
Plasmid#234080PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertaeBlue
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3228
Plasmid#234090PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertaeBlue
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3223
Plasmid#234085PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInserteforCP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3393
Plasmid#234077PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInserteforCP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225590PurposeAAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSADB19-dTomato Barcode Library
Pooled Library#227671PurposeRVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of SADB19 strain G-deleted rabies virus.DepositorExpressionMammalianSpeciesSyntheticAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_hi
Plasmid#128850PurposeHigh copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only