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Plasmid#51972Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actinDepositorInsertNano-lantern
UseTagsActinExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-Cb5-pEGFP-C1
Plasmid#166764PurposeTo target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.DepositorInsertCb5 tail anchor (Cyb5a Rat)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Hygro c1
Plasmid#54463DepositorInserthygromycin phosphotranferase (hptII) (pCAMBIA)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708/710A
Plasmid#70153Purposemammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutationsDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationS708A, S710APromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
LI C-D mCherry c1
Plasmid#54418DepositorInsertmCherry
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1650-1706_P
Plasmid#147250PurposeMammalian Expression of HsXRN1_1650-1706DepositorInsertHsXRN1_1650-1706 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1174-1649_AH
Plasmid#148903PurposeMammalian Expression of HsXRN1_1174-1649DepositorInsertHsXRN1_1174-1649 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Clavesin2 (pEGFP-C1-Sec14b)
Plasmid#47420DepositorInsertClavesin2 (CLVS2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D GFP c1
Plasmid#54414DepositorInsertsGFP
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Slp2-a E11A/R32A
Plasmid#40055DepositorInsertSlp2-a E11A/R32A (Sytl2 Mouse)
UseTagsEGFPExpressionMammalianMutationGlutamate 11 to Alanine, Arginine 32 to AlaninePromoterCMV promoterAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
LI C-D Cerulean c1
Plasmid#54417DepositorInsertCFP Cerulean
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a K>Q
Plasmid#40044DepositorInserthSlp4-a K>Q (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationLysine 410,412,416,564,566,571, 572 to GlutaminePromoterCMV promoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-5-4a
Plasmid#40045DepositorInserthSlp4-5-4a (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationSlp4 linker substituted by Slp5 linker domain (s…PromoterCMV promoterAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-IRSp53(trunc C1)
Plasmid#221273PurposeExpression of IRSp53 C-terminal truncation 1 (247-355) in mammalian cells by retroviral transductionDepositorInsertIRSp53 C-terminal truncation 1 (247-355) (Baiap2 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-GMIP(trunc C1)
Plasmid#221279PurposeExpression of GMIP C-terminal truncation 1 (1-64) in mammalian cells by retroviral transductionDepositorInsertGMIP C-terminal truncation 1 (1-64) (Gmip Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-631-894
Plasmid#220089PurposeTruncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsNeonGreen-GGSGGExpressionMammalianMutationamino acids 631-894PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail
Plasmid#220079PurposeSAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorInsertSACM1l-C389S-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationC389SdeltaTMDPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-FKBPflex-ELL-CAAX
Plasmid#220076Purposean extra long linker attached to a CAAX domain with an mCherry tagDepositorInsertCAAX (HRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-FKBPflex-OSBP-siRNAr
Plasmid#220066PurposesiRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophoreDepositorInsertOSBPL5 siRNA resistant (OSBPL5 Human)
UseTagsExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only