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Plasmid#162611PurposeAllows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-mCerulean3-cpVenus[E172]
Plasmid#125203PurposeSB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high controlDepositorInsertmCerulean3-cpVenus[E172]
UseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G
Plasmid#18016DepositorInsertFLIPglu D154A Delta13V Venus-Y67G
UseTagsECFP and Venus-Y67GExpressionMammalianMutationmglB-D154A; non-functional VenusPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC
Plasmid#69583PurposeExpresses p53 tagged with mKate2 and split C-term mVenusDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa455-550)
Plasmid#189556PurposeProtein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 455-550PromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hADAM10(aa220-459)
Plasmid#189555PurposeProtein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 220-459PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa551-654)
Plasmid#189557PurposeProtein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa551-654PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hADAM10(aa455-654)
Plasmid#189561PurposeProtein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 455-654PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
Plasmid#52903PurposeBinary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertReg-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN
Plasmid#69582PurposeExpresses p53 tagged with mKate2 and split N-term mVenusDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
NC4 pCDNA3.1 VENUS WTX (1-804)
Plasmid#36959DepositorInsertWTX (AMER1 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hSFRP1(aa53-169)
Plasmid#189546PurposeProtein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.DepositorInserttruncated secreted frizzled related protein 1 (SFRP1 Human)
UseTagsVFExpressionMammalianMutationcontains aa53-169PromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a His tag-FRB-Venus C
Plasmid#100980PurposeEncodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from BacteriaDepositorInsertHis-FRB-Venus C
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-pA_U6-BbsI (pE057)
Plasmid#149351PurposeMammalian expression vector for VenusDepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC1-mCherry-H2B-Venus
Plasmid#129559Purposenuclear markerDepositorInsertmcherry and Histone 2B fused to venus
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-G13-G12
Plasmid#25742PurposeLentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.DepositorInsertG alpha 13 and 12 miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
Plasmid#69586PurposeExpresses mutated p53-L344P tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-FRB-Venus-173-N
Plasmid#87615PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.DepositorInsertLyn-FRB-Venus173-N
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only