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Plasmid#216541PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2DepositorInsertPTK2 (S732A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S732APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS910A
Plasmid#216542PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2DepositorInsertPTK2 (S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S910APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5’
Plasmid#114140PurposeMammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIMDepositorInsertAKAR5' C-terminally tagged by KRas C tail
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
R725-X09-635: His6-tev-Hs.SPRED1(13-125)
Plasmid#159581PurposeBaculovirus protein expression of His6-tev-Hs.SPRED1(13-125)DepositorInsertHis6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKP712/713A
Plasmid#216544PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2DepositorInsertPTK2 (P712/713A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation P712/713APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS732A/S910A
Plasmid#221642PurposeThis retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2DepositorInsertPTK2 (S732A/S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK double mutation S732A/S910A (dominant negativ…PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKR177/178A
Plasmid#216543PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2DepositorInsertPTK2 (R177/178A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation R177/178APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5
Plasmid#114139PurposeMammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 C-terminally tagged by KRas C tail
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on puromycin-shMTH1
Plasmid#110918PurposeTo knock down MTH1 (Mut T Homolog 1) after addition of doxyclineDepositorInsertMTH1 (NUDT1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorInsertHis6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRMPVneo.Yap.3093
Plasmid#59915PurposeTet on shRNA for conditional Yap1 knockdownDepositorInsertYap1 (Yap1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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7432 Bicistronic_GFP_ires_puro
Plasmid#64336PurposeThis a retroviral expression plasmid expressing GFP along with puro resistance geneDepositorInsertGFP
UseRetroviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
7749 Bicistronic_ires_hygro
Plasmid#64374PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance geneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8200 Bicistronic_GFP_ires_hygro
Plasmid#64375PurposeThis a retroviral expression plasmid expressing GFP along with hygro resistance geneDepositorInsertEGFP
UseRetroviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
6691 Bicistronic_ires_puro
Plasmid#64335PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance geneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJV126
Plasmid#124250PurposeIn vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV127
Plasmid#124251PurposeIn vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -