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Plasmid#180549PurposeEntry vector containing gfasPurpleDepositorInsertgfasPurple
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No8
Plasmid#194902PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.8
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY91
Plasmid#191084Purposeglr-4 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertglr-4 (glr-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA
Plasmid#194725Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFBDepositorArticleUseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-GLRX-roGFP2
Plasmid#194288PurposeMultisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQFDepositorInsertGLRX-roGFP2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY89
Plasmid#191082Purposeglc-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertglc-3 (glc-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY83
Plasmid#191078Purposeflp-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertflp-8 (flp-8 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY78
Plasmid#191073Purposedop-6 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertdop-6 (dop-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY65
Plasmid#191062Purposebas-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertbas-1 (bas-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-STf
Plasmid#181998PurposeCMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell linesDepositorInsertH2B-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mini-talin P229S (headR13DD P229S)
Plasmid#182995Purposeexpression of minitalin P229S in mammalian cellsDepositorInsertTalin 1 (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationP229SPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
talin1-head P229S
Plasmid#182997Purposeexpression of talin1 head P229S in mammalian cellsDepositorInserttalin1 head P229S (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationP229SPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY82
Plasmid#191077Purposeflp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertflp-7 (flp-7 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY86
Plasmid#191079Purposegcy-25 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-25 (gcy-25 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY87
Plasmid#191080Purposegcy-27 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-27 (gcy-27 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY88
Plasmid#191081Purposeggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertggr-1
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY90
Plasmid#191083Purposeglr-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertglr-2 (glr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY92
Plasmid#191085Purposeglr-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertglr-8 (glr-8 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only