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Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.2
Plasmid#149431PurposeminiTdg gene, SUMOylation mutantDepositorInsertTdg (Tdg Mouse)
UseCre/LoxTagsExpressionMammalianMutationK341A SUMOylation mutant, SNPs of Tdg gene in OLA…PromoterTdg promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-FOXA1
Plasmid#139304PurposeKnocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1DepositorInsertshRNA and amiRNA against FOXA1
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND4_L17_S24_S11_S19_S13
Plasmid#132732PurposeT7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13DepositorInsert3WJ_NAND4_L17_S24_S11_S19_S13
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG_Ptet*_ToeRep_N01_switch
Plasmid#132737PurposeInducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_ToeRep_N01_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_Ptet*_3WJrep_N19_switch
Plasmid#132739PurposeInducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_3WJrep_N19_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND3_L11_S11_S13_S19
Plasmid#132731PurposeT7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19DepositorInsert3WJ_NAND3_L11_S11_S13_S19
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1-ATG7 3'UTR(406)
Plasmid#122070PurposeExpressing ATG1 with ATG7 3'UTRDepositorInsertserine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native 3'UTR was replaced by ATG7 3'…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD5cmyc2-bglpA
Plasmid#110645PurposeExpression plasmid for c-Myc-tag embedded into human CD52 with short polyADepositorInsertCMV-CDcmyc2-bglpA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-ScSEIPIN-Ndelta
Plasmid#96985PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
UseTags6xHis and GFPExpressionPlantMutationN-terminal 39 nucleotides were deleted from yeast…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN2
Plasmid#96987PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsExpressionYeastMutationPromoterPhosphoglycerate Kinase (PGK) promoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3N-1C
Plasmid#96983PurposeExpress mutated Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides from the N terminus…Promoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN1N-3C
Plasmid#96982PurposeExpress mutated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationA segment of 108 nucleotides from the N terminus…Promoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc
Plasmid#91935PurposeBacterial expression of maize SUMO1a and inactive SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc
Plasmid#91933PurposeBacterial expression of maize SUMO1a and inactive SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-mTmod3-150-352_domain
Plasmid#70755Purposemouse Tropomodulin3, 150-352 domain for bacterial expressionDepositorInsertTropomodulin3 150-352 AA fragment (Tmod3 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4F04
Plasmid#71030PurposeGateway entry cloneDepositorInsertSin3A (Sin3A Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF1B10
Plasmid#70840PurposeGateway entry cloneDepositorInsertSce (Sce Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only