Addgene

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Showing: 6741 - 6760 of 24635 results

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Use

Tags

His and thrombin cleavage

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mCer

Plasmid

#185603

Purpose

Lentiviral construct for expression of mCerulean targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mCer

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV hSynapsin SV-tag

Plasmid

#163685

Purpose

express SV-Tag construct in a Cre-independent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synatophysin 9X HA tag T2A tdtomato

Use

Tags

9X HA

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIRSTD)-mNG

Plasmid

#189721

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-mCer

Plasmid

#185602

Purpose

Lentiviral construct for expression of mCerulean targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-mCer

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV hSynapsin flexed SV-tag

Plasmid

#163686

Purpose

express SV-Tag construct in a Cre-dependent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synaptophysin 9X HA

Use

Tags

9X HA

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJCX4

Plasmid

#170756

Purpose

Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Use

Tags

Mxe GyrA intein - chitin binding domain (CBD)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-CUP1-His-Ubi

Plasmid

#99541

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-mnx1

Plasmid

#82402

Purpose

Gateway-compatible zebrafish mnx1 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mnx1 promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G178E

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
MCV-HF

Plasmid

#32057

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-HF genome

Use

Tags

Expression

Mutation

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3-ST

Plasmid

#129262

Purpose

Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3-ST

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

Plasmid

#107247

Purpose

beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

Depositor

Bryan Roth

Article

Roth et al bioRxiv 251769

Insert

ARRB2

Use

AAV

Tags

MYC, PYLcs, and YFP

Expression

Mutation

Promoter

hSYN

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-lynD3cpV

Plasmid

#37472

Depositor

Roger Tsien, Amy Palmer

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Use

Tags

the myristoylation and palmitoyla- tion sequence …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4092694

Plasmid

#153907

Purpose

Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Bacterial

Mutation

wt

Promoter

Promoter | t7 consensus from NEB, G is +1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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