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Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GAS41
Plasmid#15428DepositorInsertGAS41 (Yeats4 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Mito-CaNARi
Plasmid#64851PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNARi
UseTagsCerulean, N-terminal 30 amino acids from DAKAP1, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80R
Plasmid#118350PurposePlasmid expresses hHSF1 with K80 in DBD mutated to R.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK80RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-mKate2
Plasmid#67671PurposeMultisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2DepositorInsertAPEX2-P2A-mKate2
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5077 pcDNA4c hBrd4 (1-594)
Plasmid#14453DepositorInsertBrd4 (1-594) (BRD4 Human)
UseTagsHisExpressionMammalianMutationamino acids 1-594PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJA3
Plasmid#19493DepositorInsertDNAJA3 (DNAJA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
CFP_Gem_pcDNA4_HisMaxC
Plasmid#41653DepositorInsertGem, GTP binding proteins (RGK) (GEM Human)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HSPH3
Plasmid#19455DepositorInsertheat shock 70kDa protein 4-like (HSPA4L Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-150
Plasmid#26310DepositorInserthsa-mir-150 (MIR150 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 mmu-mir-133a-1
Plasmid#26326DepositorInsertmmu-mir-133a-1 (Mir133a-1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense
Plasmid#46835PurposeExpress cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
UseTags6xHisExpressionYeastMutationG951A,C957APromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDNA3-Cerulean-FHA1-NES
Plasmid#138202PurposeEncodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.DepositorInsertCerulean-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-plasma membrane/pcDNA3
Plasmid#51975Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membraneDepositorInsertNano-lantern
UseTagsHIS and LynExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-Kras
Plasmid#118408PurposePlasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-Kras
UseTagsC-terminal targeting sequence from KrasExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-ECFP-GIT1
Plasmid#15223DepositorInsertG protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
UseTagsECFP and flagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 SV8
Plasmid#184011PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).DepositorInsertProm1 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationWild type cDNAPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev(T/A)
Plasmid#118479PurposeNegative-control mutant for RAB-AKARev biosensor.DepositorInsertRAB-AKARev(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only