Addgene

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pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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lacI-PT5-gusA-pBAV1k

Plasmid

#30501

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pGCS-C3m(3xFLAG)

Plasmid

#85726

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCEV-G3-Ph

Plasmid

#46818

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and GAL10

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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LSL

Plasmid

#66590

Purpose

Lox-STOP-Lox reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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MyD116.PFLAG.CMV2

Plasmid

#21834

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 (Ppp1r15a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) VSFP2.1

Plasmid

#16255

Depositor

Thomas Knopfel

Article

Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Insert

VSFP2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pTHSSe_43

Plasmid

#109241

Purpose

pSC101 var1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMT Lifeact-TagBFP

Plasmid

#126664

Purpose

Inducible expression of Lifeact-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Lifeact-TagBFP fusion

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKmK.P3

Plasmid

#125036

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

ENO1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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iGFP

Plasmid

#66589

Purpose

inverted GFP reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pMevB

Plasmid

#17819

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
lacI-PT7-gusA-pBAV1k

Plasmid

#30504

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucoronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S28 histone 3 phosphorylation reporter-NLS

Plasmid

#32787

Depositor

Alice Ting

Article

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3.3TOPO-T7-hCas9

Plasmid

#161876

Purpose

In vitro transcription of SpCas9

Depositor

Stephane Pelletier

Article

Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

Insert

human codon optimized SpCas9

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-EsaI

Plasmid

#47660

Purpose

esaI gene downstream of esaR in pAC-EsaR (CmR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

esaI-term

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pET51b_HCAII

Plasmid

#187110

Purpose

Expression of HCAII in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

HCAII

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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