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Plasmid#172574PurposeFor gateway cloning of N-terminal tagged TARG1 constructs.DepositorInsertTARG1 (OARD1 Human)
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xHA-Syn-smFP-flag
Plasmid#182563PurposeFor mouse Nav1.6 knockin with 3xHA at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xHA donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-rScn2a-3xV5-Syn-miRFP670
Plasmid#182566PurposeFor rat Nav1.2 knockin with 3xV5 at the extracellular loopDepositorInsertsrat Scn2a gRNA and 3xV5 donor DNA
miRFP670
UseAAV and CRISPRTagsExpressionMutationPromoterSyn and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-HaloTag-V5-EF1-sfGFP
Plasmid#182565PurposeFor mouse Nav1.6 knockin with HaloTag-V5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xV5-EF1-smFP-flag
Plasmid#182562PurposeFor mouse Nav1.6 knockin with 3xV5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry
Plasmid#182560PurposeExpresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherryDepositorInsertsmouse Scn8a ICL1
sfGFP-P2A-mCherry
UseTagsExpressionMammalianMutationPromoterSynAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin_AD
Plasmid#148531PurposeMammalian Expression of DmRoquinDepositorInsertDmRoquin (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
UseTags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN1-W378AW437A_O
Plasmid#147136PurposeInsect Expression of CeAIN1-W378AW437ADepositorInsertCeAIN1-W378AW437A (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1_1-350_O
Plasmid#147122PurposeInsect Expression of CeAIN1_1-350DepositorInsertCeAIN1_1-350 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-DFDF-KFKF_C
Plasmid#146036PurposeInsect Expression of DmEDC3-DFDF-KFKFDepositorInsertDmEDC3-DFDF-KFKF (Edc3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-FDF-ADA_C
Plasmid#146037PurposeInsect Expression of DmEDC3-FDF-ADADepositorInsertDmEDC3-FDF-ADA (Edc3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA
Plasmid#170768PurposeMethylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coliDepositorInsertsM.XhoI
M.MunI
M.Ocy1ORF8430P
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-ccDockerin
Plasmid#72200PurposeMBP-ccDockerin expression plasmidDepositorInsertClostridium Cellulolyticum Type I Dockerin
UseTagsHis-Tag and Maltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0136
Plasmid#178398PurposeKSHV Kaposinclouster_RevCompl-KapATGdel_SynDepositorInsertKaposinclouster_RevCompl-KapATGdel_Syn
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0136MR
Plasmid#178399PurposeKSHV Kaposinclouster_RevCompl-KapATGdel_SynDepositorInsertKaposinclouster_RevCompl-KapATGdel_Syn
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-PML peptide
Plasmid#175247PurposeBacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycinDepositorInsertPML (PML Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only