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Plasmid#207675PurposeTrio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterCymRC, PhlF, VanCCAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only
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pHYX137
Plasmid#202814PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX138
Plasmid#202815PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterScADH2Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX143
Plasmid#202816PurposeControl plasmid expressing mScarlet-I fluorescent protein.DepositorInsertmScarlet-I
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX173
Plasmid#202817PurposePlasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.DepositorInsertTEV protease
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN1_EF1a-tNGFR-2A-Puro
Plasmid#200553PurposeDual barcoding cassettes for SSN-seq; Barcode Set 1DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN2_EF1a-tNGFR-2A-Puro
Plasmid#201112PurposeDual barcoding cassettes for SSN-seq; Barcode Set 2DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN3_EF1a-tNGFR-2A-Puro
Plasmid#201113PurposeDual barcoding cassettes for SSN-seq; Barcode Set 3DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN4_EF1a-tNGFR-2A-Puro
Plasmid#201114PurposeDual barcoding cassettes for SSN-seq; Barcode Set 4DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN5_EF1a-tNGFR-2A-Puro
Plasmid#201115PurposeDual barcoding cassettes for SSN-seq; Barcode Set 5DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN6_EF1a-tNGFR-2A-Puro
Plasmid#201116PurposeDual barcoding cassettes for SSN-seq; Barcode Set 6DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN7_EF1a-tNGFR-2A-Puro
Plasmid#201117PurposeDual barcoding cassettes for SSN-seq; Barcode Set 7DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN8_EF1a-tNGFR-2A-Puro
Plasmid#201118PurposeDual barcoding cassettes for SSN-seq; Barcode Set 8DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgTS40
Plasmid#169630PurposepX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.DepositorInsertU6-driven gRNA expression and PCAG-driven SpCas9 expression
UseTagsExpressionMammalianMutationPromoterU6 / PCAGAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1041
Plasmid#169619PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH228-Tier1-OVanO2-PCMVmin2-SEAP
Plasmid#169564PurposeTier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).DepositorInsertvanillic acid-controlled SEAP production
UseTagsExpressionMammalianMutationPromoterVanO2-PCMVminAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1040
Plasmid#169618PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1039
Plasmid#169617PurposeTier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2365-Tier1-PhCMV-TetR
Plasmid#169566PurposeTier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).DepositorInsertPCMV-driven tetracycline repressor protein class B from transposon Tn10
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP
Plasmid#169588PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-2Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only