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Plasmid#199196PurposeInsert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochond…ExpressionMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-S-LexA::p65
Plasmid#133564PurposeSwap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-I-LexA::p65
Plasmid#133563PurposeSwap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-D-LexA::p65
Plasmid#133562PurposeSwap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFPV25-cR-2iG
Plasmid#204618PurposeFluorescent reporter for SPI2 induction in SalmonellaDepositorInsertsrpsM promoter
sseA promoter
UseTagsGFP and dsRedExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ir7f-T2A-QF2 HDR plasmid
Plasmid#140942PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f geneDepositorInsertIr7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
UseTagsExpressionMutation4 point mutations (annotated on plasmid map) were…PromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF-DBD
Plasmid#162525PurposePlasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2HYG
Plasmid#30170DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsdsRed2ExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-4gRNA-GBX2-RFP
Plasmid#192288PurposeExpresses RFP and four sgRNAs against GBX2DepositorInsertRFP and four sgRNA GBX2 (GBX2 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)
Plasmid#110143PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseBinary vectorTagscogDsRedExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTRmiRc2
Plasmid#25754PurposeEntry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.DepositorTypeEmpty backboneUseEntry vectorTagsdsRed2ExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEN_TRmiRc2
Plasmid#25750PurposeEntry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.DepositorTypeEmpty backboneUseEntry vectorTagsdsRed2ExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pHairyRed
Plasmid#69709PurposeFluorescent reporter of Agrobacterium rhizogenes mediated transformed rootsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBRN168-MXMT
Plasmid#79819PurposeDsREDmonomer-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsRN168/DsREDmonomer (1–168)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-DXMT
Plasmid#79822PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRN168-DXMT
Plasmid#79821PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRN168/DsREDmonomer (1–168)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-MXMT
Plasmid#79820PurposeDsREDmonomer-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only