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Plasmid#139308PurposeN-terminal half of split PI-PLC targeted to plasma membraneDepositorInsertLYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-tagBFP
Plasmid#65254Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI-NLS-VP16
Plasmid#103837PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInserttagBFP-LacI-NLS-VP16
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)
Plasmid#128174PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-TagBFP
Plasmid#117783PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Trex2-bpA-EF1BFP
Plasmid#111144PurposeExpression of mouse Trex2 and BFP reporter in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCAG and EF1Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-gcry-TagBFP
Plasmid#117789PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)
Plasmid#128171PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mCherry-ApoB-TagBFP
Plasmid#129383PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFPDepositorInsertmCherry-ApoB-Tag-BFP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-TagBFP
Plasmid#117792PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W
Plasmid#163138PurposeLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tagDepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98976PurposeCas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(N863A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-EBFP2
Plasmid#158553PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mTagBFP2 L1-R5
Plasmid#84388PurposeMultisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmTagBFP2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Scara3-IRES-BFP
Plasmid#117265PurposeRetroviral overexpression of Scara3DepositorInsertScara3 (Scara3 Mouse)
UseRetroviralTagsExpressionMammalianMutationSee depositor comments below.PromoterLTRAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CarO-BFP2-TSERex
Plasmid#124795PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertCarO-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Char-BFP2-TSERex
Plasmid#124792PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertChar-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Mac-BFP2-TSERex
Plasmid#124793PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertMac-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only