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Showing: 641 - 660 of 754 results

  1. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid
    #154141
    Purpose
    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Article
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Use
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Mutation
    R887E
    Promoter
    SFFV
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154141-plasmid-map-sequence-id-345617

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  2. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

    Plasmid
    #211904
    Purpose
    Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.
    Depositor
    Yannick Doyon
    Article
    Levesque et al bioRxiv 2023.09.14.557485
    Insert
    CD19-CAR-28z-2A-EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    211904-plasmid-map-sequence-id-420112

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  5. pACBE-ASR (pXK-607)

    Plasmid
    #208822
    Purpose
    Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.
    Depositor
    Kun Xu
    Article
    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi
    Type
    Empty backbone
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208822-plasmid-map-sequence-id-417248

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  14. Plas-gRNA-Z3EV

    Plasmid
    #157659
    Purpose
    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.
    Depositor
    Frank Albert
    Article
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Insert
    GFP-gRNA NatMX Z3EV
    Use
    Insert storage (replicative in e. coli)
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    157659-plasmid-map-sequence-id-309102

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  19. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  20. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197065
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    I3-01
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197065-plasmid-map-sequence-id-402601

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Showing: 641 - 660 of 754 results