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Plasmid#172746PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-16
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-mCherry-FRB
Plasmid#172443PurposeExpression of plasma membrane protein Stargazin tagged with mCherry-FRBDepositorInsertCacng2 (Cacng2 Mouse)
UseTagsmCherry-HA-FRBExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-T.2-ABE8e
Plasmid#185669PurposeExpresses eNme2-T.2-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-T.2
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/rh10
Plasmid#112866PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap-rh10
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald
Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV5)
Viral Prep#114471-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMF-TetTight
Plasmid#193288PurposeLentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgeneDepositorInsertrtTA-P2A-tTS
UseLentiviralTagsExpressionMammalianMutationPromoterEF‐1αAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mScarlet-I
Plasmid#98818PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsertmts
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYSEAP
Plasmid#37326DepositorInsertSEAP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYSEAP
Plasmid#37326DepositorInsertSEAP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_TauFRET_3xKQ_wpre
Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2ExpressionMutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP_CD47_SU
Plasmid#65473PurposeGFP tagged CD47 with its own short 3'UTRDepositorInsertsSignal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIV-dTomato
Plasmid#21374Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomatoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHIV-dTomato
Plasmid#21374Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomatoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A44
Plasmid#132042PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A44 (SLC25A44 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-MAP4-N-10
Plasmid#54153PurposeLocalization: Microtubules, Excitation: 487, Emission: 509DepositorInsertMAP4 (MAP4 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVoPo-04
Plasmid#187839PurposeSuicide vector to generate markerless gene deletions in Fusobacterium nucleatumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only