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Plasmid#210909PurposeInsect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF3B:D127-E814 (EIF3B Human)
UseTagsMHHHHHHSSGRENLYFQGMQExpressionInsectMutationY760C sequence conflictPromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_EML4:250-877
Plasmid#210914PurposeInsect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.DepositorInsertEML4:I250-A877 (EML4 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationK283E natural variant (VAR_031726), I382V Natural…PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_EIF3I:1-325
Plasmid#210910PurposeInsect expression of full-length human EIF3I. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF3I:M1-A325 (EIF3I Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_LRRK2:2141-2527
Plasmid#210899PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_COP1:386-731
Plasmid#210887PurposeInsect expression of human COP1 fragment A386 to V731. N-terminal 6X His tag with TEV cleavage.DepositorInsertCOP1:A386-V731 (COP1 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CDC20:161-477
Plasmid#210943PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.DepositorInsertCDC20:S161-P477 (CDC20 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC_LRRK2:2141-2527
Plasmid#210900PurposeInsect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
UseTagsAENLYFQSHHHHHHHHHHDYKDDDDK and MExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterP10 and CMVAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_hsHAUScomplex_FL
Plasmid#202203PurposeBaculoviral transfer vector to co-express 8 subunits of human augmin complexDepositorInsertsHAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
UseTags-mGFP-TEV-TwinStrep and His-tag (6x)ExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only