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Plasmid#13642DepositorInsertToll-Like Receptor 9 (TLR9 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-ERCreER-4x6T
Plasmid#196411PurposeAstrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertERT2-Cre-ERT2
UseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_FL
Plasmid#139114Purposeexpress FL hnRNPF with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 KBTBD4 R313PRR
Plasmid#184626PurposeDoxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cellsDepositorInsertN-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationR313PRRPromoterTRE promoter, Tet ONAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-H2A
Plasmid#36207PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDS1902
Plasmid#167278PurposeExpression plasmid for Candida albicans containing a red-shifted firefly luciferaseDepositorInsertred shifted luciferase
UseTagsExpressionMutationPromoterACT1Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E293 Hs.RPS6KA3
Plasmid#70577PurposeGateway ORF clone of human RPS6KA3 [NM_004586.2] with stop codon (for native or N-terminal fusions)DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2939
Plasmid#144415PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXA6 (HOXA6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2481
Plasmid#142948PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXD4 (MXD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0805
Plasmid#141710PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMARCC2 (SMARCC2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorInsertABE8.8-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
CN2256-rAAV-eHGT_462m-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#164456PurposeDirect-expressing SYFP2 AAV VirusInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-Halo
Plasmid#129592PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B2.0
Plasmid#167158PurposeGolden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mNeon-TurboID
Plasmid#186610PurposeThird generation lentiviral vector expressing mNeonGreen fused to V5-TurboIDDepositorInsertmNeon-V5-TurboID
UseLentiviralTagsmNeonGreenExpressionMammalianMutationFor reference see Branon et al., 2018PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic cpVenus control
Plasmid#186788PurposeIdentical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertcytoplasmic cpVenus control
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-GLUT4-7myc-GFP
Plasmid#52872Purposedetection and measurement of GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 with human c-terminus, Rat)
UseRetroviralTags7x-myc (internal) and GFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only