-
Plasmid#51702Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 127-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-281)
Plasmid#51700Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 282-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
7M8
Plasmid#64839PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.DepositorInsert7M8 cap
UseAAVTagsExpressionMutation7mer insertion in the AAV2 capPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP-shTACC3
Plasmid#59356PurposeKnocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG ChR2 E123T T159C 2A tDimer
Plasmid#85399PurposeHigh efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoterDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterCAG and ribosomal skip sequence 2AAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVTagsExpressionMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-FKBP-TACC3-shTACC3
Plasmid#59354PurposeKnocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsEGFP and FKBPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB3
Plasmid#131159PurposeRetroviral expression of flag-tagged mouse TRIB3DepositorInsertTrib3 (Trib3 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(delta JBD)
Plasmid#51767Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 126-263PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
PASK gRNA (BRDN0001145672)
Plasmid#76203Purpose3rd generation lentiviral gRNA plasmid targeting human PASKDepositorInsertPASK (PASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b K294R
Plasmid#118359PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PASK gRNA (BRDN0001149035)
Plasmid#76204Purpose3rd generation lentiviral gRNA plasmid targeting human PASKDepositorInsertPASK (PASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PASK gRNA (BRDN0001148005)
Plasmid#76205Purpose3rd generation lentiviral gRNA plasmid targeting human PASKDepositorInsertPASK (PASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S and C699T)
Plasmid#52132Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470 and changed C478S and C6…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-470)
Plasmid#52125Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281 and 471-707PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7Jip1(282-470)
Plasmid#51703Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 1-281 and 471-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertmTQ2=mturquoise2 blue fluorescent gene
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMutationPromoterVillinAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhGC wt T2A tDimer
Plasmid#101720Purposehumanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the mDepositorInsertsCatRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhSyn1 and ribosomal skip sequence T2AAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
aP2-GFPtpz
Plasmid#114211PurposeaP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophageDepositorInsertaP2 promoter + pOB4 + eGFPtopaz (Fabp4 Mouse, Aequorea victoria, Synthetic)
UseTagsNoneExpressionMammalianMutationPromoteraP2/Fabp4Available sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only