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pcDNA3.1-MS2-HA-HshnRNPR_AA
Plasmid#148235PurposeMammalian Expression of HshnRNPRDepositorInsertHshnRNPR (HNRNPR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS263
Plasmid#122379PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC46A1
Plasmid#132191PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC46A1 (SLC46A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pPGS-hSNAIL.fl.flag
Plasmid#25695DepositorInsertSNAIL (SNAI1 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCGN-S2P-WT
Plasmid#32957PurposeFor mammalian expression of human S2P with an HA tagDepositorInsertS2P (MBTPS2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHA AKT2 T309A/S474A
Plasmid#60128Purposeexpresses a mutant AKT2DepositorInsertprotein kinase B (AKT2 Human)
UseRetroviralTagsHAExpressionMutationSerine 474 and Threonine 309 changed to alaninesPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-G719A
Plasmid#116253PurposeLentiviral expression of EGFR G719ADepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationG719APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
myr-palm_mCherry2-mEGFP
Plasmid#127816PurposeHetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signalDepositorInsertmyristoylated-palmitoylated-mCherry2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1183
Plasmid#119254Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
2PH-PLCd-APEX2-P2A-mKate2
Plasmid#71284PurposeMammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporterDepositorInsert2PH-PLCd-APEX2-P2A-mKate2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGC4593
Plasmid#110206PurposeAccessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)DepositorInsertsTEV protease
aaRS
UseTagsExpressionBacterialMutationPromoterPbad and PtetAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
SLC9A3R2-TwinStrepII
Plasmid#83200PurposeExpression of tagged protein in mammalian cellsDepositorInsertSLC9A3R2 (Slc9a3r2 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC4A11
Plasmid#132192PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC4A11 (SLC4A11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b-Caspase-3(D3A,V266E)
Plasmid#90091PurposeExpresses caspase D3A and V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9A V266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-ccdB chr I
Plasmid#41894DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPDAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P33
Plasmid#120018PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dynamin-I-K44A-6His/Flag
Plasmid#32696DepositorInsertDynamin I (Dnm1 Rat)
UseTagsFlag and His6ExpressionMammalianMutationK44APromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only
