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Plasmid#67571PurposeExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cellsDepositorInsertA-kinase anchoring protein Lbc (AKAP13 Human)
UseTagsFLAG and GFPExpressionMammalianMutationR2604G in AKAPPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralTagsExpressionMutationWTPromoterU6Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dSaCas9-KRAB-gRNA-TRE-blast
Plasmid#201151PurposeLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsSadCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAExpressionMutationgRNA sequence: ATCAGTGATAGAGAACGTATGPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP (AAV5)
Viral Prep#55641-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Flp-dependent)Available sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH
Plasmid#209294PurposemitoBE expression vector, target mtRNR2 gene.DepositorInsertSOD2-3xHA-TALE(RNR2-Left)-MutH
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60229PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianMutationPromoterCBh and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro
Plasmid#138152PurposeExpresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleusDepositorInserteGFP-MCS
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3
Plasmid#164695PurposeExpression of PRMT3 with an N-terminal FLAG tagDepositorInsertPRMT3 (PRMT3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-3xFLAG-V5
Plasmid#87071PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseLentiviralTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo
Plasmid#75088Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a-Cas9-2XNES-2ERT2
Plasmid#120551PurposeExpress Cas9, 2xNES and 2 tandem ERT2 in mammalian cellsDepositorInsertCas9, NES and ERT2
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 (AAV1)
Viral Prep#100839-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 (#100839). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPGN-K
Plasmid#174579PurposepOPIN-GG expression vector compatible with N-terminal tagging, Kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorInsertHDAC7 (HDAC7 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
hBax C3-EGFP
Plasmid#19741DepositorInsertBax (BAX Human)
UseTagsGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDAC435
Plasmid#195238PurposeMammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-NLS-RfxCas13d-mcherry-NLS-Flag
Plasmid#165077Purposeoverexpression of RfxCas13d in human cellsDepositorInsertRfxCas13d
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only