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Plasmid#171252PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser650Ala (AR-W741C-S650A)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S650D
Plasmid#171253PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser650Asp (AR-W741C-S650D)PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cn-VA-NHEJ1-S262E/S263E
Plasmid#141342PurposeLentiviral vector for expression of human NHEJ1-S262E/S263E in mammalian cellsDepositorInsertNHEJ1-S262E/S263E (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianMutationS262E/S263EPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cn-VA-NHEJ1-S262A/S263A
Plasmid#141343PurposeLentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cellsDepositorInsertNHEJ1-S262A/S263A (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianMutationS262A/S263APromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEPT_Cdk2-T160A-siR
Plasmid#73975PurposeHDR template to generate Cdk2-T160A-siRDepositorUseAAVTagsExpressionMutationT160 mutated to Ala and siRNA target site mutatedPromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-KKKAAA
Plasmid#171219PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.DepositorInsertAR-KKKAAA (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K630A-K632A-K633APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D
Plasmid#177320PurposeLentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).DepositorInsertKRAS 4B (KRAS Human)
UseLentiviralTagsFKBP-derived dimerization domain (DD, or DmrB) an…ExpressionMammalianMutationG12DPromoterTetOn-CMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEPT_Cdk2-T160E-siR
Plasmid#73976PurposeHDR template to generate Cdk2-T160E-siRDepositorUseAAVTagsExpressionMutationT160 mutated to Glu and siRNA target site mutatedPromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-WT_VA
Plasmid#141327PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cellsDepositorInsertTARDBP-WT (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIS-MDH1-HIS
Plasmid#184558PurposeBacterial expression of human MDH1 with HIS TagDepositorInsertMalate dehydrogenase 1 (MDH1 Human)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-A315T_VA
Plasmid#141329PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cellsDepositorInsertTARDBP-A315T (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationA315TPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-G368A_VA
Plasmid#141330PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-G368A in mammalian cellsDepositorInsertTARDBP-G368A (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationG368APromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-G287A_VA
Plasmid#141328PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-G287A in mammalian cellsDepositorInsertTARDBP-G287A (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationG287APromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-W385G_VA
Plasmid#141331PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-W385G in mammalian cellsDepositorInsertTARDBP-W385G (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationW385GPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - C2 (UCSF)
Plasmid#169209PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iM
Plasmid#58973PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iG
Plasmid#58975PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNICBio2_WDR5:2-334
Plasmid#211027Purposebacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.DepositorInsertWDR5:A2-C334 (WDR5 Human)
UseTagsMHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationwild typePromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only