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Plasmid#205150PurposeExpress UbC-driven FKRP and EF-1a driven BSDDepositorInsertFukutin-related protein (FKRP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1845
Plasmid#143325PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertALX4 (ALX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3507
Plasmid#144983PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNOCT (NOCT Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
BFP-Sec61 beta
Plasmid#49154Purposeexpression of Sec61 beta fused to BFP, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1346
Plasmid#143099PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF541 (ZNF541 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB1
Plasmid#63102PurposeExpression of HSPB1 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb1 (HSPB1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF3231
Plasmid#144707PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF423 (ZNF423 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1114
Plasmid#143929PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRCOR3 (RCOR3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-GFP
Plasmid#16664DepositorInserteGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
UBC plasmid
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0405
Plasmid#143212PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC8A3_STOP
Plasmid#161352PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A3 (SLC8A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC8A2_STOP
Plasmid#161340PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A2 (SLC8A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1 KBTBD4 WT
Plasmid#184624PurposeDoxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cellsDepositorInsertN-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationno mutationPromoterTRE promoter, Tet ONAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuclear cpVenus control
Plasmid#186790PurposeIdentical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertnuclear cpVenus control
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD2
Plasmid#33107DepositorInsertTEAD2 (TEAD2 Human)
UseTagsGal4ExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b-sp
Plasmid#122188PurposeBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
INCENP
Plasmid#39185PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertINCENP (INCENP Human)
UseTagsGST-TevExpressionBacterialMutationComplex with AURKBA-c004PromoterAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only