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pcdna3 T7 Jip1b

Plasmid

#52122

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pEnt R3L2 TetO(sh) mCh-Rs1-2

Plasmid

#24417

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Rs1

Use

Entry vector

Tags

FLAG and mCherry

Expression

Mutation

Human 5HT4B receptor with D100A mutation, generat…

Promoter

Available since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSBtet-2xMARS-nGFP-puro

Plasmid

#205239

Purpose

DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

Depositor

Jeremy Baskin

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Transposon

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

tight TRE promoter

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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UUUUA-pHG165c

Plasmid

#170107

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

UUUUA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MMMMA-pHG165c

Plasmid

#170108

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

MelR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA_Q182A_R287H-pHG165c

Plasmid

#170111

Purpose

Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA_Q182A_R278H

Use

Tags

Expression

Bacterial

Mutation

Q182A, R287H

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRRAA-pHG165c

Plasmid

#170112

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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LLhE_AW-pHG165

Plasmid

#90062

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhE_AW

Use

Tags

Expression

Bacterial

Mutation

I48V/Q55A/Q60R

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhS-pHG165a

Plasmid

#90040

Purpose

Constitutive expression of a chimeric LacI:GalS transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhF-pHG165a

Plasmid

#90041

Purpose

Constitutive expression of a chimeric LacI:FruR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a

Plasmid

#90042

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhR-pHG165a

Plasmid

#90043

Purpose

Constitutive expression of a chimeric LacI:RbsR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a

Plasmid

#90044

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhE-pHG165

Plasmid

#90045

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a

Plasmid

#90046

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

EE

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 Flag Jip1b

Plasmid

#52123

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-AldoA

Plasmid

#224925

Purpose

Inducible expression of human aldolase A

Depositor

Liskin Swint-Kruse

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

Aldolase A (ALDOA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

David Schaffer, John Flannery

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Tags

Expression

Mutation

I319V, N451D, D532N

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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