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Addgene
Showing: 6441 - 6460 of 16077 results

  1. p667-UBC-GFP-V5_IDG-K

    Plasmid
    #135242
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135242-plasmid-map-sequence-id-270199

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  2. p663-UBC-V5-dsRed_IDG-K

    Plasmid
    #135262
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135262-plasmid-map-sequence-id-268083

    Enlarge

  3. p663-UBC-V5-eGFP_IDG-K

    Plasmid
    #135265
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135265-plasmid-map-sequence-id-268086

    Enlarge

  4. WDR5 (3UR4)

    Plasmid
    #36900
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. 2QNR (NEDD5)

    Plasmid
    #101273
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD5 (SEPTIN2 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101273-plasmid-map-sequence-id-193507

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  6. pIRES-H3.3-WT-FLAG-BFP

    Plasmid
    #206139
    Purpose
    Expression of a FLAG tagged human histone H3.3 gene with BFP
    Depositor
    Maria Castro
    Insert
    H3.3 (H3-3A Human)
    Use
    Tags
    FLAG-eBFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206139-plasmid-map-sequence-id-405576

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  9. PP-MAPK2 (3RP9)

    Plasmid
    #32862
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MAPK2
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acids 123-548 only, P421L
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. TDRD5

    Plasmid
    #32869
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD5 (TDRD5 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. PANK1

    Plasmid
    #32871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 231-597 only
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. MLH1

    Plasmid
    #28120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-347.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pcDNA3.1-E-Flag

    Plasmid
    #101858
    Purpose
    Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    101858-plasmid-map-sequence-id-211082

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  15. TBXTA-c027

    Plasmid
    #139764
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139764-plasmid-map-sequence-id-296577

    Enlarge

  16. TBXTA-c028

    Plasmid
    #139765
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139765-plasmid-map-sequence-id-296580

    Enlarge

  17. TBXTA-c043

    Plasmid
    #139766
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41- D225
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139766-plasmid-map-sequence-id-296581

    Enlarge

  18. TBXTA-c044

    Plasmid
    #139767
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41- D225
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139767-plasmid-map-sequence-id-296582

    Enlarge

  19. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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Showing: 6441 - 6460 of 16077 results