-
Plasmid#168486PurposeMammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM0
Plasmid#59125PurposeFAK fret control sensor without kinase domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM0
Plasmid#59125PurposeFAK fret control sensor without kinase domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
-
RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
C239-E03: attB2r-IRES-ffLuc2-pA-attB3
Plasmid#162919PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase with upstream IRES sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C239-E03: attB2r-IRES-ffLuc2-pA-attB3
Plasmid#162919PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase with upstream IRES sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
RNAPII_CTD27-37
Plasmid#98686PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 27-37, residues 1773-1852PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-37
Plasmid#98686PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 27-37, residues 1773-1852PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT100-neo
Plasmid#123137PurposeGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertNeomycin, poly-A terminator
UseSynthetic Biology; Golden gate donor vectorTagsExpressionMutationPromoternoneAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT100-neo
Plasmid#123137PurposeGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertNeomycin, poly-A terminator
UseSynthetic Biology; Golden gate donor vectorTagsExpressionMutationPromoternoneAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p224 pCMV-RFP/CREM
Plasmid#8401DepositorInsertRFP/CREM
UseCre/LoxTagsExpressionMammalianMutationmodified Cre, LOX 511 sites surrounding RFP withi…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
p224 pCMV-RFP/CREM
Plasmid#8401DepositorInsertRFP/CREM
UseCre/LoxTagsExpressionMammalianMutationmodified Cre, LOX 511 sites surrounding RFP withi…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (773-908)
Plasmid#1316DepositorInsertHsp104 (773-908) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationdeletion of aa1-773PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (361-548)
Plasmid#1317DepositorInsertHsp104 (361-548) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa361-548 of Hsp104PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (773-908)
Plasmid#1316DepositorInsertHsp104 (773-908) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationdeletion of aa1-773PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only