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Showing: 6421 - 6440 of 6570 results

  1. pRegev_S5.49C>A

    Plasmid
    #204175
    Purpose
    pRegev_S5 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S5 with 49C>A mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    49C>A mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204175-plasmid-map-sequence-id-405426

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  2. pRegev_D4

    Plasmid
    #204184
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    D4 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204184-plasmid-map-sequence-id-405407

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  3. pRegev_D2

    Plasmid
    #204180
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D2 in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    61C>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204180-plasmid-map-sequence-id-405406

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  4. pRegev_S5.49C>A.59G>U

    Plasmid
    #204177
    Purpose
    pRegev_S5 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    49C>A and 59G>U mutations
    Promoter
    Truncated CAG
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204177-plasmid-map-sequence-id-404686

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  9. 2xMARS-nGFP

    Plasmid
    #205235
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205235-plasmid-map-sequence-id-404746

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  10. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108867-plasmid-map-sequence-id-209739

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  15. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  17. pHB4

    Plasmid
    #53462
    Purpose
    This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.
    Depositor
    Elizabeth Conibear
    Article
    Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11
    Insert
    yeGFP-Snc1-Suc2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    53462-plasmid-map-sequence-id-83347

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  19. MARS-nGFP

    Plasmid
    #205234
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    Use
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205234-plasmid-map-sequence-id-404747

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  20. pCDNA3-2xMARS-PLD

    Plasmid
    #205237
    Purpose
    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
    Depositor
    Jeremy Baskin
    Inserts
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    Use
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205237-plasmid-map-sequence-id-404742

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Showing: 6421 - 6440 of 6570 results