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Plasmid#162687PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
UseTagsHisExpressionBacterialMutationdeltaG251-T472, C224H, C529F; codon optimized for…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ217
Plasmid#162685PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
UseTagsHisExpressionBacterialMutationG489C, S530C; codon optimized for E. coli K12PromoterT7/lacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK122
Plasmid#74295PurposeProduces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusionDepositorInserthypothetical acetic acid resistance protein B
UseTagsNusAExpressionBacterialMutationPromoterT7Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATCH1
Plasmid#39274DepositorInsertUpdated MCS for pET vectors with enhanced stop codons
UseTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
KIF3A406
Plasmid#129768PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKIF3A (Kif3a Mouse)
UseTagsHis6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCoofy21
Plasmid#43979DepositorTypeEmpty backboneUseTagsHis10-OneStrepExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy33
Plasmid#44001DepositorTypeEmpty backboneUseTagsHis10 and OneStrepExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1
Plasmid#43974DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy4
Plasmid#43986DepositorTypeEmpty backboneUseTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy3
Plasmid#43983DepositorTypeEmpty backboneUseTagsHis6-GSTExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy22
Plasmid#43992DepositorTypeEmpty backboneUseTagsHis10 and SExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy2
Plasmid#43981DepositorTypeEmpty backboneUseTagsTrx-His6ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy6
Plasmid#43990DepositorTypeEmpty backboneUseTagsHis6-Sumo3ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy16
Plasmid#43988DepositorTypeEmpty backboneUseTagsHis10-NusAExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
KSP_K560GFP
Plasmid#129770PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKSP (kif11.L Frog)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy37
Plasmid#43998DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy25
Plasmid#43997DepositorTypeEmpty backboneUseTagsHalo and His10ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy23
Plasmid#43996DepositorTypeEmpty backboneUseTagsHis10 and TrxExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy38
Plasmid#43982DepositorTypeEmpty backboneUseTagsTrx-His10ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only