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Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM30
Plasmid#133673PurposeIntegration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.DepositorInsertp1k-ERG6-GFP (ERG6 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
3521 pET15b Bid p15
Plasmid#8782DepositorInsertBid p15 (Bid Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-Siwi
Plasmid#50560PurposeExpresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cellsDepositorInsertSiwi (Aubergine )
UseTags6xHIS and FLAGExpressionInsectMutationPromoterOpIE2Available sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
NID1-bio-His
Plasmid#52068PurposeExpresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNID1 (NID1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_S100A11_S-100
Plasmid#109804PurposeProtein expression and purification of S100A11_S-100DepositorInsertS100A11_S-100 (S100A11 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHES941
Plasmid#87942PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorInsertSmpd4 (Smpd4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-NES
Plasmid#118407PurposeCytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-NES
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RAD51_Rad51
Plasmid#110031PurposeProtein expression and purification of RAD51_Rad51DepositorInsertRAD51_Rad51 (RAD51 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mCherry-GAI(1-92)
Plasmid#37316DepositorInsertGAI (GAI Mustard Weed)
UseTagsTom20 and mCherryExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human-4xUAS
Plasmid#125150PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-Sec16S
Plasmid#15775DepositorInsertSec16S (SEC16B Human)
UseTagsmEGFPExpressionMammalianMutationN/APromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OptoPB
Plasmid#113935PurposeExpress a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactionsDepositorInsertOptoPB
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR(T/A)
Plasmid#118469PurposeNegative-control mutant for sapphireCKAR biosensor.DepositorInsertsapphireCKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)
Plasmid#164040PurposeExpression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2DepositorInsertsgTS5-sgTS6-sgTS7
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM135
Plasmid#133659PurposeIntegration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-mir124a
Plasmid#61539PurposeExpresses mir124a in mammalian cellsDepositorInsertmir124a (MIR124-1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only