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Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneUseTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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371-Bc
Plasmid#97028PurposeyORF-tev-His6DepositorTypeEmpty backboneUseTagsHis6 tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWO-SUMO-3C_14M_zTDP2
Plasmid#200512PurposeExpression of zebrafish TDP2 recombinant proteinDepositorInsertTDP2 (tdp2b Zebrafish)
UseTagsHIS-SUMOExpressionBacterialMutationL136N, A139S, C239T, K240R, K309Q, K314L, T315G, …PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
371-T
Plasmid#97035PurposeHis6-Thioredoxin-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, TEV cleavage site, and Thioredoxin funs…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-R
Plasmid#97033PurposeStrepII-tev-yORFDepositorTypeEmpty backboneUseTagsStrepII tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-S
Plasmid#97034PurposeHis6-Sumo-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, SUMO domain, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-O
Plasmid#97032PurposeHis6-Mocr-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, TEV cleavage site, and mOCR fusionExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-L
Plasmid#97031PurposeFLAG-tev-yORFDepositorTypeEmpty backboneUseTagsFLAG tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-B
Plasmid#97027PurposeHis6-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
thoc5.018.389
Plasmid#137266PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertthoc5 (THOC5 Human)
UseTags(His)6-TEVExpressionBacterialMutation018-389PromoterAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
NKAP.001.140
Plasmid#137206PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertNKAP (NKAP Human)
UseTags(His)6-TEVExpressionBacterialMutation001-140PromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ207
Plasmid#162680PurposepET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ199
Plasmid#162672PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
UseTagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201819PurposeA plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoterDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseTagsExpressionMammalianMutationPromoterpOTTC1751Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1Q1
Plasmid#97326PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1Q1
UseTags6xHis - TEVExpressionBacterialMutationAddition of Q residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAPN2A-c002
Plasmid#205104PurposeCAPN2 protein expressionDepositorInsertCAPN2 (CAPN2 Human)
UseTags6His-TEV and AviTagExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.169.S34E
Plasmid#137643PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTags(His)6-TEVExpressionBacterialMutation001-169-S34EPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.211.522.gst
Plasmid#137650PurposeExpresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTagsGST-(His)6-thrombinExpressionBacterialMutation211-522-gstPromoterAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only