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Plasmid#73757PurposeNabi2.213 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.213
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK0_012
Plasmid#123967PurposeEncodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-BxBI landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NIR-FbLAG16
Plasmid#220749PurposeNIR-FbLAG16 expression in mammalian cells. pAAV vector.DepositorInsertNIR-FbLAG16
UseAAV and Affinity Reagent/ AntibodyTagsExpressionMutationPromoterCAGAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NIR-FbLAG30
Plasmid#220750PurposeNIR-FbLAG30 expression in mammalian cells. pAAV vector.DepositorInsertNIR-FbLAG30
UseAAV and Affinity Reagent/ AntibodyTagsExpressionMutationPromoterCAGAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationFour Arg to Ala mutations in zinc finger backbonePromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RCC
Plasmid#190635PurposeMammalian expression of genetically encoded calcium indicator RCCDepositorInsertRCC
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSWD95Y14D
Plasmid#159098PurposeCckA FRET Sensor with cyclic-di-GMP point mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationMutated Y514 to D514PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_011
Plasmid#123966PurposeEncodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-PhiC31 landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Pansio
Plasmid#214445PurposeExpresses eSpCas9 and gRNA targeting Pansio-1 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Keppel
Plasmid#214446PurposeExpresses eSpCas9 and gRNA targeting Keppel-19 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Olônne
Plasmid#214447PurposeExpresses eSpCas9 and gRNA targeting Olônne-18 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorInsertGFP11-RhoA (RHOA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ad:2CMV_A2R2-N25K3
Plasmid#122242PurposeExpresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backboneDepositorUseAdenoviralTagsSignal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (AAV1)
Viral Prep#98928-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (#98928). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of Ruby2sm-Flag spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby2sm-Flag (spaghetti monster construct)Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Diatom uLoop Assembly Kit
Plasmid Kit#1000000238PurposeThe collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.DepositorAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid Kit#1000000119PurposeUsed for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated productionDepositorAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only