Addgene

We narrowed to 3,847 results for: SPL

Showing: 621 - 640 of 3847 results

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pRegev_D3

Plasmid

#204182

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D1.61C>G

Plasmid

#204179

Purpose

pRegev_D1 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D1 with 61C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

61C>G mutation

Promoter

Truncated CAG

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D2.30C>G

Plasmid

#204181

Purpose

pRegev_D2 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D2 with 30C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

30C>G mutation

Promoter

Truncated CAG

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D4.34C>G

Plasmid

#204185

Purpose

pRegev_D4 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D4 with 34C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

34C>G mutation

Promoter

Truncated CAG

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3.48C>G

Plasmid

#204183

Purpose

pRegev_D3 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3 with 48C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

48C>G mutation

Promoter

Truncated CAG

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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SPELL-GDI Component 2

Plasmid

#170487

Purpose

SPELL GDI1 C-lobe fused to FRB. Split site is 66

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB-GDI1(C-lobe)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-GDI Component 1

Plasmid

#170486

Purpose

SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

GDI1(N-lobe)-iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD217

Plasmid

#160939

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mTagBFP2-HA Tag

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) short BP-3'SS

Plasmid

#111333

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Use

Tags

Expression

Bacterial

Mutation

shorter branch to 3' splice site distance

Promoter

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M1

Plasmid

#106321

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQ-->AAAA

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M5

Plasmid

#106325

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc splice site insertion mutant EEKQNA-->EEK…

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M4

Plasmid

#106324

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc splice site deletion mutant EEKQNA-->EEKNA

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M3

Plasmid

#106323

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQNAV-->AAAAAAA

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSABAD14-750

Plasmid

#45616

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

MjaTFIIBC53

Use

Tags

GB1-H6

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMetYC-Dest

Plasmid

#105081

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

Cub-PLV

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Plasmid

#52985

Purpose

split Venus attached to MCP and PCP respectively

Depositor

Robert Singer

Article

Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

Insert

nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

ubc

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pTomatoSlicer

Plasmid

#221085

Purpose

Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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