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Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmGamillus
Plasmid#182971PurposeStably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmGamillus
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP1-loxP-bar-lox2272-71
Plasmid#168785PurposeVector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP2-loxP-bar-lox2272-71
Plasmid#168786PurposeVector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
IS1 Homology arm 1
IS1 Homology arm 2
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolB(T304I)-Puro
Plasmid#177143PurposeLentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0007
Plasmid#102839PurposeBinary vector containing Sr22 PI573523 allele driven by Sr33 promoter, Sr33 terminatorDepositorInsertSr22 PI573523 (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterSr33 promoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0002
Plasmid#102834PurposeBinary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr33 terminatorDepositorInsertSr22 Schomburgk (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterSr33 promoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0003
Plasmid#102835PurposeBinary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr22 terminatorDepositorInsertSr22 Schomburgk (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterSr33 promoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0004
Plasmid#102836PurposeBinary vector containing Sr22 PI190945 allele driven by Sr33 promoter, Sr33 terminatorDepositorInsertSr22 PI190945 (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterSr33 promoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0005
Plasmid#102837PurposeBinary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminatorDepositorInsertSr22 PI573523 (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterMaize ubiquitinAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW_0006
Plasmid#102838PurposeBinary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr33 promoter (undomesticated)DepositorInsertSr22 Schomburgk (NEWENTRY Synthetic)
UseTagsExpressionPlantMutationPromoterSr33 promoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMAL-c2X-ORF24-NTD-3L_A
Plasmid#138466PurposeExpresses MBP-tagged ORF24-NTD 3L_A in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201; amino acids L73-75 mutated to …PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-2xCTD
Plasmid#138470PurposeExpresses GST-tagged 2xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation2x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A_L75A-3xFLAG
Plasmid#138429PurposeExpresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L73, L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L74A_L75A-3xFLAG
Plasmid#138430PurposeExpresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L74, L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R68A-3xFLAG
Plasmid#138431PurposeExpresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R68 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R72A-3xFLAG
Plasmid#138432PurposeExpresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R72 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only