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Plasmid#79602PurposeFUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sitesDepositorInsertd2GFP + miR-200 family targets
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt1
Plasmid#35905DepositorInsertWnt1 (WNT1 Human)
UseTagsExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548DepositorInsertHA-hM4D
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC008 - pBR322 with tetR-inducible RFP
Plasmid#79157Purposeinducible RFP expression vectorDepositorInsertTetR + RFP
UseTagsExpressionMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-SpCas9-NG
Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TagGFP2
Plasmid#121426PurposeTAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertempty TagGFP2
UseLentiviralTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbV5
Plasmid#201476PurposeNanoBit detection of V5-tagged proteinDepositorInsertLgBit-NbV5
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4 DD
Plasmid#26999DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationC867APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK1035
Plasmid#8832DepositorInsertTVMV protease
UseTagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCX-mutSpiCee
Plasmid#140903Purposemammalian expression of a mutated form of SpiCee, that does not affect calcium signalingDepositorInsertmutSpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Skm1-PH
Plasmid#71371Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertSkm1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa1-132PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls
Plasmid#64112PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_HP1
Plasmid#82607PurposeExpresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cellsDepositorInsertsfCherry2(11)_HP1b
UseTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only