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Addgene
Showing: 6341 - 6360 of 16077 results
  1. pSL1423

    Plasmid
    #194904
    Purpose
    pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum
    Depositor
    Insert
    TurboID
    Use
    Plasmodium falciparum
    Tags
    GFPmut2
    Expression
    Mutation
    Promoter
    None. This allows a C-terminal fusion to a protei…
    Availability
    Academic Institutions and Nonprofits only
  2. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. HOXD4-WV-MSCV

    Plasmid
    #21002
    Depositor
    Insert
    HOXD4 (Hoxd4 Mouse)
    Use
    Retroviral
    Tags
    His and T7-tag
    Expression
    Mammalian
    Mutation
    W->V PBX1 interaction mutant
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pLenti puro HA-Ubiquitin

    Plasmid
    #74218
    Purpose
    Lentiviral expression of HA tagged Ubiquitin
    Depositor
    Insert
    ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  7. pDsred monomer hygro C1- MLPH

    Plasmid
    #89231
    Purpose
    Expression of tagged wild type human Melanophilin with Dsred monomer in mammalian cells
    Depositor
    Insert
    Human Melanophilin (MLPH Human)
    Use
    Tags
    Dsred monomer
    Expression
    Mammalian
    Mutation
    wild type
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
  9. FLAG-Ex1-17-deltaP1-PTPN22

    Plasmid
    #195388
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 domain) with FLAG tag
    Depositor
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17 and lacking…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. FLAG-Ex1-17-deltaP2-PTPN22

    Plasmid
    #195395
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P2 domain) with FLAG tag
    Depositor
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17 and lacking…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid
    #78336
    Purpose
    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag
    Insert
    PAX3 (PAX3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Availability
    Academic Institutions and Nonprofits only
  12. pINDUCER20-FLAG-hPAX7 (#734)

    Plasmid
    #78339
    Purpose
    Doxycycline-inducible lentiviral overexpression of human PAX7 ORF with a FLAG tag
    Insert
    PAX7 (PAX7 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Availability
    Academic Institutions and Nonprofits only
  13. RF-88-Ate1

    Plasmid
    #79187
    Purpose
    Expression of C-terminally Flag tagged ATE1 in mammalian cells
    Depositor
    Insert
    ATE1 (ATE1 Human)
    Use
    Tags
    3x Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  14. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    USP21 (USP21 Human)
    Use
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. USP08 (3N3K)

    Plasmid
    #28115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    USP08 (USP8 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    Catalytic domain only.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. MPP7

    Plasmid
    #28149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    membrane protein, palmitoylated 7 (MPP7 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    PDZ Domain (residues 135-225)
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  17. UBH14

    Plasmid
    #28158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    E3 ligase UHRF2 (UHRF2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    SRA Domain (residues 419-648)
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. TPK1

    Plasmid
    #32865
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    TPK1 (TPK1 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6341 - 6360 of 16077 results