Addgene

We narrowed to 29,354 results for: uros

Showing: 6321 - 6340 of 29354 results

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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

F23S A123V I195F

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-FLEX-rev-ChR2-tdtomato

Plasmid

#18917

Purpose

Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-tdtomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdtomato

Expression

Mutation

Promoter

CAG

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (no Cre)

Plasmid

#200370

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDC191

Plasmid

#115792

Purpose

Expresses Rat synaptobrevin 2

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Synaptobrevin2 (Vamp2 Rat)

Use

Tags

hexa histidine

Expression

Bacterial

Mutation

S28C and C103S (please see comments below)

Promoter

T7

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pPRIME-dsRed-shscramble

Plasmid

#71384

Purpose

Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

CMV promoter-dsRed-mir30-shscramble

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer/promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBT2dR3-proB

Plasmid

#122595

Purpose

BE PACE selection, express T7 RNA polymerase with degron-GTCCA target

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

T7 RNA polymerase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (hSyn-Cre) backbone

Plasmid

#200368

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF0

Plasmid

#200371

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLX307 hcRed1

Plasmid

#117732

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF2

Plasmid

#200373

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pXL004-ishRNA-scramble

Plasmid

#36311

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of scramble

Use

Lentiviral and RNAi

Tags

EF1a-TetR-IRES-RFP-Puro

Expression

Mammalian

Mutation

Promoter

TetO-H1

Available since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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FU- dio Teal-gephyrin - W

Plasmid

#73918

Purpose

To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Teal-gephyrin

Use

Lentiviral

Tags

Teal

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Expression

Mutation

H134R

Promoter

CAG

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-HA-RINT1

Plasmid

#39841

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

RINT1 (RINT1 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pDEST-FRT/T0-GFP-PALB2

Plasmid

#71113

Purpose

mammalian expession of GFP-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-mCherry-LacR-PALB2

Plasmid

#71112

Purpose

mammalian expession of mCherry-LacR-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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